Comparison of standard mismatch repair deficiency and microsatellite instability tests in a large cancer series.

ABSTRACT:

Background

The tumor-agnostic indication of immune checkpoint inhibitors to treat cancers with mismatch repair deficiency (dMMR)/microsatellite instability (MSI) increased the demand for such tests beyond Lynch syndrome. International guideline recommendations accept immunohistochemistry (IHC) for dMMR or molecular techniques (PCR or NGS) for MSI status determinations considering the two tests are equal, although there are scattered reports contradicting to this presumption.

Materials and methods

Here we have directly compared four protein MMR immunohistochemistry (IHC) to MSI Pentaplex PCR test in a large cancer patient cohort (n = 1306) of our diagnostic center where the two tests have been run parallel in 703 cases.

Results

In this study we have found a high discre

SUBMITTER: Nadorvari ML 

PROVIDER: S-EPMC10863158 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature