Characterization of two distinct immortalized endothelial cell lines, EA.hy926 and HMEC-1, for in vitro studies: exploring the impact of calcium electroporation, Ca²⁺ signaling and transcriptomic profiles.

ABSTRACT:

Background

Disruption of Ca²⁺ homeostasis after calcium electroporation (CaEP) in tumors has been shown to elicit an enhanced antitumor effect with varying impacts on healthy tissue, such as endothelium. Therefore, our study aimed to determine differences in Ca²⁺ kinetics and gene expression involved in the regulation of Ca²⁺ signaling and homeostasis, as well as effects of CaEP on cytoskeleton and adherens junctions of the established endothelial cell lines EA.hy926 and HMEC-1.

Methods

CaEP was performed on EA.hy926 and HMEC-1 cells with increasing Ca²⁺ concentrations. Viability after CaEP was assessed using Presto Blue, while the effect on cytoskeleton and adherens junctions was evaluated via immunofluorescence staining (F-actin,

SUBMITTER: Lisec B 

PROVIDER: S-EPMC10863159 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature