A Genoproteomic Approach to Detect Peptide Markers of Bacterial Respiratory Pathogens.

ABSTRACT:

Background

Rapid identification of respiratory pathogens may facilitate targeted antimicrobial therapy. Direct identification of bacteria in bronchoalveolar lavage (BAL) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is confounded by interfering substances. We describe a method to identify unique peptide markers of 5 gram-negative bacteria by liquid chromatography-tandem mass spectrometry (LC-MS/MS) for direct pathogen identification in BAL.

Methods

In silico translation and digestion were performed on 14-25 whole genomes representing strains of Acinetobacter baumannii, Moraxella catarrhalis, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Klebsiella pneumoniae. Peptides constituting theoretical co

SUBMITTER: Wang H 

PROVIDER: S-EPMC10863334 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature