Microneme-located VP2 in Eimeria acervulina elicits effective protective immunity against infectious bursal disease virus.

ABSTRACT: Using transgenic Eimeria spp. to deliver exogenous antigens is a viable option for developing multivalent live vaccines. Previous research revealed that the location of antigen expression in recombinant Eimeria dictates the magnitude and type of immune responses. In this study, we constructed genetically modified Eimeria acervulina that expressed VP2 protein, a protective antigen from infectious bursal disease virus (IBDV), on the surface or in the microneme of sporozoites. After vaccination, VP2-specific antibody was readily detected in specific pathogen-free chickens receiving transgenic E. acervulina parasites expressing VP2 in microneme, but animals vaccinated with which expressing VP2 on surface failed to produce detectable antibody after two times immuniza

SUBMITTER: Yu Y 

PROVIDER: S-EPMC10863409 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature