Metabolic diversity of human macrophages: potential influence on Staphylococcus aureus intracellular survival.

ABSTRACT: Staphylococcus aureus is a leading cause of medical device-associated biofilm infections. This is influenced by the ability of S. aureus biofilm to evade the host immune response, which is partially driven by the anti-inflammatory cytokine interleukin-10 (IL-10). Here, we show that treatment of human monocyte-derived macrophages (HMDMs) with IL-10 enhanced biofilm formation, suggesting that macrophage anti-inflammatory programming likely plays an important role during the transition from planktonic to biofilm growth. To identify S. aureus genes that were important for intracellular survival in HMDMs and how this was affected by IL-10, transposon sequencing was performed. The size of the S. aureus essential genome was similar between unstimulated HMDMs and the ou

SUBMITTER: Bertrand BP 

PROVIDER: S-EPMC10863412 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature