Mass Spectrometry-Based Multi-omics Integration with a Single Set of C. elegans Samples.

ABSTRACT: Mass spectrometry-based large-scale multi-omics research has proven to be powerful in answering biological questions; nonetheless, it faces many challenges from sample preparation to downstream data integration. To efficiently extract biomolecules of different physicochemical properties, preparation of various sample type needs specific tailoring, especially of difficult ones, such as Caenorhabditis elegans. In this study, we sought to develop a multi-omics sample preparation method starting with a single set ofC. elegans samples to save time, minimize variability, expand biomolecule coverage, and promote multi-omics integration. We investigated tissue disruption methods to effectively release biomolecules and optimized extraction strategies to achieve broader and more reprod

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC10863427 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature