Metabolic conditioning of CD8⁺ effector T cells for adoptive cell therapy.

ABSTRACT: CD8⁺ effector T (T_E) cell proliferation and cytokine production depends on enhanced glucose metabolism. However, circulating T cells continuously adapt to glucose fluctuations caused by diet and inter-organ metabolite exchange. Here we show that transient glucose restriction (TGR) in activated CD8⁺ T_E cells metabolically primes effector functions and enhances tumour clearance in mice. Tumour-specific TGR CD8⁺ T_E cells co-cultured with tumour spheroids in replete conditions display enhanced effector molecule expression, and adoptive transfer of these cells in a murine lymphoma model leads to greater numbers of immunologically functional circulating donor cells and complete tumour clearance. Mechanistically, T_E cell

SUBMITTER: Klein Geltink RI 

PROVIDER: S-EPMC10863625 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature