Vortex fluidic regulated phospholipid equilibria involving liposomes down to sub-micelle size assemblies.

ABSTRACT: Conventional channel-based microfluidic platforms have gained prominence in controlling the bottom-up formation of phospholipid based nanostructures including liposomes. However, there are challenges in the production of liposomes from rapidly scalable processes. These have been overcome using a vortex fluidic device (VFD), which is a thin film microfluidic platform rather than channel-based, affording ∼110 nm diameter liposomes. The high yielding and high throughput continuous flow process has a 45° tilted rapidly rotating glass tube with an inner hydrophobic surface. Processing is also possible in the confined mode of operation which is effective for labelling pre-VFD-prepared liposomes with fluorophore tags for subsequent mechanistic studies on the fate of liposomes under shear stress i

SUBMITTER: Joseph N 

PROVIDER: S-EPMC10863723 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature