Comprehensive Molecular Genetic Analysis in Glioma Patients by Next Generation Sequencing.

ABSTRACT:

Background

Glioma is caused by multiple genomic alterations. The evolving classification of gliomas emphasizes the significance of molecular testing. Next generation sequencing (NGS) offers the assessment of parallel combinations of multiple genetic alterations and identifying actionable mutations that guide treatment. This study comprehensively analyzed glioma patients using multi-gene NGS panels, providing powerful insights to inform diagnostic classification and targeted therapies.

Methods

We conducted a targeted panel-based NGS analysis on formalin-fixed and paraffin-embedded nucleic acids extracted from a total of 147 glioma patients. These samples underwent amplicon capture-based library preparation and sequenced using the Oncomine Comprehensive Assay platform. The res

SUBMITTER: Kim T 

PROVIDER: S-EPMC10864139 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature