Fecal supernatants from dogs with idiopathic epilepsy activate enteric neurons.

ABSTRACT:

Introduction

Alterations in the composition and function of the gut microbiome have been reported in idiopathic epilepsy (IE), however, interactions of gut microbes with the enteric nervous system (ENS) in this context require further study. This pilot study examined how gastrointestinal microbiota (GIM), their metabolites, and nutrients contained in intestinal contents communicate with the ENS.

Methods

Fecal supernatants (FS) from healthy dogs and dogs with IE, including drug-naïve, phenobarbital (PB) responsive, and PB non-responsive dogs, were applied to cultured myenteric neurons to test their activation using voltage-sensitive dye neuroimaging. Additionally, the concentrations of short-chain fatty acids (SCFAs) in the FS were quantified.

Results

Our findings indi

SUBMITTER: Elfers K 

PROVIDER: S-EPMC10864448 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature