Unknown

Dataset Information

0

Antibiofilm and antivirulence activities of laminarin-gold nanoparticles in standard and host-mimicking media.


ABSTRACT: The rapidly rising antimicrobial resistance (AMR) in pathogenic bacteria has become one of the most serious public health challenges, with a high death rate. Most pathogenic bacteria have been recognized as a source of AMR and a primary barrier to antimicrobial treatment failure due to the development of biofilms and the production of virulence factors. In this work, nanotechnology was employed as a substitute method to control the formation of biofilms and attenuate virulence features in Pseudomonas aeruginosa and Staphylococcus aureus. We synthesized biocompatible gold nanoparticles from marine-derived laminarin as potential biofilm and virulence treatments. Laminarin-gold nanoparticles (Lam-AuNPs) have been identified as spherical, 49.84 ± 7.32 nm in size and - 26.49 ± 1.29 mV zeta potential. The MIC value of Lam-AuNPs against several drug-resistant microbial pathogens varied from 2 to 1024 μg/mL in both standard and host-mimicking media. Sub-MIC values of Lam-AuNPs were reported to effectively reduce the production of P. aeruginosa and S. aureus biofilms in both standard and host-mimicking growth media. Furthermore, the sub-MIC of Lam-AuNPs strongly reduced hemolysis, pyocyanin, pyoverdine, protease, and several forms of flagellar and pili-mediated motility in P. aeruginosa. Lam-AuNPs also inhibited S. aureus hemolysis and the production of amyloid fibrils. The Lam-AuNPs strongly dispersed the preformed mature biofilm of these pathogens in a dose-dependent manner. The Lam-AuNPs would be considered an alternative antibiofilm and antivirulence agent to control P. aeruginosa and S. aureus infections. KEY POINTS: • Lam-AuNPs were biosynthesized to control biofilm and virulence. • Lam-AuNPs show effective biofilm inhibition in standard and host-mimicking media. • Lam-AuNPs suppress various virulence factors of P. aeruginosa and S. aureus.

SUBMITTER: Tabassum N 

PROVIDER: S-EPMC10864539 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

Antibiofilm and antivirulence activities of laminarin-gold nanoparticles in standard and host-mimicking media.

Tabassum Nazia N   Khan Fazlurrahman F   Jeong Geum-Jae GJ   Oh Dokyung D   Kim Young-Mog YM  

Applied microbiology and biotechnology 20240213 1


The rapidly rising antimicrobial resistance (AMR) in pathogenic bacteria has become one of the most serious public health challenges, with a high death rate. Most pathogenic bacteria have been recognized as a source of AMR and a primary barrier to antimicrobial treatment failure due to the development of biofilms and the production of virulence factors. In this work, nanotechnology was employed as a substitute method to control the formation of biofilms and attenuate virulence features in Pseudo  ...[more]

Similar Datasets

| S-EPMC9686622 | biostudies-literature
| S-EPMC11851435 | biostudies-literature
| S-EPMC8144575 | biostudies-literature
| S-EPMC10966193 | biostudies-literature
2018-12-25 | GSE117201 | GEO
| S-EPMC9419574 | biostudies-literature
| S-EPMC9960922 | biostudies-literature
| S-EPMC6121036 | biostudies-literature
| S-EPMC8782275 | biostudies-literature
| S-EPMC7148532 | biostudies-literature