Effect of Exosomes Derived from Bone Marrow Mesenchymal Stem Cells on Ovarian Granulosa Cells of Immature NMRI Mice.

ABSTRACT:

Objective

In recent years, in vitro maturation (IVM) has become the focus of fertility maintenance, and infertility treatment. The aim of this study is development of oocytes during folliculogenesis and oogenesis is greatly influenced by the presence of BMP-7, BMP-15, and GDF-9 genes, which are present in exosomes generated from bone marrow stem cells.

Materials and methods

In the experimental study, we investigated how exosomes obtained from bone marrow stem cells affected development and expansion of ovarian granulosa cells (GCs) in NMRI mice. In this in vitro experiment, bone marrow stem cells were isolated from mice's bone marrow, and after identification, exosomes were recovered. Exosome doses of 100, 50, and 25 μg/ml were applied to GCs before usin

SUBMITTER: Farrokhyar S 

PROVIDER: S-EPMC10864772 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature