Revealing splenectomy-driven microRNA hsa-7b-5p's role in pancreatic cancer progression.

ABSTRACT: Splenectomy often accompanies distal pancreatectomy for pancreatic cancer. However, debates persist on splenic function loss impact. Prior studies in mice revealed splenectomy promotes pancreatic cancer growth by altering CD4/Foxp3 and CD8/Foxp3 ratios. The effect on other immune cells remains unclear. Clinical observations indicate splenectomy induces immunosuppression, heightening recurrence and metastasis risk. Here, we established an orthotopic pancreatic cancer model with splenectomy and observed a significant increase in tumor burden. Flow cytometry revealed elevated MDSCs, CD8+PD-1high+ T cells, and reduced CD4⁺ T cells, CD8⁺ T cells, and natural killer cells in tumors. Bulk sequencing identified increased MicroRNA (miRNA) hsa-7b-5p post-splenectomy, correlatin

SUBMITTER: Wu L 

PROVIDER: S-EPMC10864800 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature