In vitro and in vivo models define a molecular signature reference for human embryonic notochordal cells.

ABSTRACT: Understanding the emergence of human notochordal cells (NC) is essential for the development of regenerative approaches. We present a comprehensive investigation into the specification and generation of bona fide NC using a straightforward pluripotent stem cell (PSC)-based system benchmarked with human fetal notochord. By integrating in vitro and in vivo transcriptomic data at single-cell resolution, we establish an extended molecular signature and overcome the limitations associated with studying human notochordal lineage at early developmental stages. We show that TGF-β inhibition enhances the yield and homogeneity of notochordal lineage commitment in vitro. Furthermore, this study characterizes regulators of cell-fate decision and matrisome enriched in the no

SUBMITTER: Warin J 

PROVIDER: S-EPMC10865399 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature