The application of a 4D-printed chitosan-based stem cell carrier for the repair of corneal alkali burns.

ABSTRACT:

Background

Corneal alkali burns can lead to ulceration, perforation, and even corneal blindness due to epithelial defects and extensive cell necrosis, resulting in poor healing outcomes. Previous studies have found that chitosan-based in situ hydrogel loaded with limbal epithelium stem cells (LESCs) has a certain reparative effect on corneal alkali burns. However, the inconsistent pore sizes of the carriers and low cell loading rates have resulted in suboptimal repair outcomes. In this study, 4D bioprinting technology was used to prepare a chitosan-based thermosensitive gel carrier (4D-CTH) with uniform pore size and adjustable shape to improve the transfer capacity of LESCs.

Methods

Prepare solutions of chitosan acetate, carboxymethyl chitosan, and β-glycerophosphate sodium

SUBMITTER: Wang Z 

PROVIDER: S-EPMC10865625 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature