Increased CpG methylation at the CDH1 locus in inflamed ileal mucosa of patients with Crohn disease.

ABSTRACT:

Background

E-cadherin, a major actor of cell adhesion in the intestinal barrier, is encoded by the CDH1 gene associated with susceptibility to Crohn Disease (CD) and colorectal cancer. Since epigenetic mechanisms are suspected to contribute to the multifactorial pathogenesis of CD, we studied CpG methylation at the CDH1 locus. The methylation of the CpG island (CGI) and of the 1st enhancer, two critical regulatory positions, was quantified in surgical specimens of inflamed ileal mucosa and in peripheral blood mononuclear cells (PBMC) of 21 CD patients. Sixteen patients operated on for a non-inflammatory bowel disease, although not normal controls, provided a macroscopically normal ileal mucosa and PBMC for comparison.

Results

In ileal mucosa, 19/21 (90%) CD patients vs 8/16

SUBMITTER: de Ponthaud C 

PROVIDER: S-EPMC10865720 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature