Computational analysis of genes with lethal knockout phenotype and prediction of essential genes in archaea.

ABSTRACT: The identification of microbial genes essential for survival as those with lethal knockout phenotype (LKP) is a common strategy for functional interrogation of genomes. However, interpretation of the LKP is complicated because a substantial fraction of the genes with this phenotype remains poorly functionally characterized. Furthermore, many genes can exhibit LKP not because their products perform essential cellular functions but because their knockout activates the toxicity of other genes (conditionally essential genes). We analyzed the sets of LKP genes for two archaea, Methanococcus maripaludis and Sulfolobus islandicus, using a variety of computational approaches aiming to differentiate between essential and conditionally essential genes and to predict at least a general

SUBMITTER: Makarova KS 

PROVIDER: S-EPMC10865827 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature