Solid-state NMR for the characterization of retinal chromophore and Schiff base in TAT rhodopsin embedded in membranes under weakly acidic conditions.

ABSTRACT: TAT rhodopsin extracted from the marine bacterium SAR11 HIMB114 has a characteristic Thr-Ala-Thr motif and contains both protonated and deprotonated states of Schiff base at physiological pH conditions due to the low pK_a. Here, using solid-state NMR spectroscopy, we investigated the ¹³C and ¹⁵N NMR signals of retinal in only the protonated state of TAT in the 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine/1-palmitoyl-2-oleoyl-sn-glycero-3-phospho (1'-rac-glycerol) (POPE/POPG) membrane at weakly acidic conditions. In the ¹³C NMR spectrum of ¹³C retinal-labeled TAT rhodopsin, the isolated 14-¹³C signals of 13-trans/15-anti and 13-cis/15-syn isomers were observed at a rati

SUBMITTER: Arikawa S 

PROVIDER: S-EPMC10865839 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature