Genetic suppressor screen identifies Tgp1 (glycerophosphocholine transporter), Kcs1 (IP₆ kinase), and Plc1 (phospholipase C) as determinants of inositol pyrophosphate toxicosis in fission yeast.

ABSTRACT: The inositol pyrophosphate signaling molecule 1,5-IP₈ is an agonist of RNA 3'-processing and transcription termination in fission yeast that regulates the expression of phosphate acquisition genes pho1, pho84, and tgp1. IP₈ is synthesized from 5-IP₇ by the Asp1 N-terminal kinase domain and catabolized by the Asp1 C-terminal pyrophosphatase domain. asp1-STF mutations that delete or inactivate the Asp1 pyrophosphatase domain elicit growth defects in yeast extract with supplements (YES) medium ranging from severe sickness to lethality. We now find that the toxicity of asp1-STF mutants is caused by a titratable constituent of yeast extract. Via a genetic screen for spontaneous suppressors, we identified a null mutation

SUBMITTER: Bednor L 

PROVIDER: S-EPMC10865970 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature