A rapid and bidirectional reporter of neural activity reveals neural correlates of social behaviors in Drosophila.

ABSTRACT: Neural activity is modulated over different timescales encompassing subseconds to hours, reflecting changes in external environment, internal state and behavior. Using Drosophila as a model, we developed a rapid and bidirectional reporter that provides a cellular readout of recent neural activity. This reporter uses nuclear versus cytoplasmic distribution of CREB-regulated transcriptional co-activator (CRTC). Subcellular distribution of GFP-tagged CRTC (CRTC::GFP) bidirectionally changes on the order of minutes and reflects both increases and decreases in neural activity. We established an automated machine-learning-based routine for efficient quantification of reporter signal. Using this reporter, we demonstrate mating-evoked activation and inactivation of modulatory neurons. We further i

SUBMITTER: Bonheur M 

PROVIDER: S-EPMC10866131 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature