Long non-coding RNA MEG3 acts as a suppressor in breast cancer by regulating miR-330/CNN1.

ABSTRACT:

Background

The current study aimed to investigate the molecular mechanism of long non-coding RNA (lncRNA) MEG3 in the development of breast cancer.

Methods

The regulating relationships among lncRNA MEG3, miRNA-330 and CNN1 were predicted by bioinformatics analysis of breast cancer samples in the Cancer Genome Atlas database. The differential expression of lncRNA MEG3, miRNA-330 and CNN1 was first validated in breast cancer tissues and cells. The effects of lncRNA MEG3 on breast cancer malignant properties were evaluated by manipulating its expression in MCF-7 and BT-474 cells. Rescue experiments, dual-luciferase assays, and RNA immunoprecipitation (RIP) experiments were further used to validate the relationships among lncRNA MEG3, miRNA-330 and CNN1.

Results

Bioinform

SUBMITTER: Yi D 

PROVIDER: S-EPMC10866439 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature