Local monomer levels and established filaments potentiate non-muscle myosin 2 assembly.

ABSTRACT: The ability to dynamically assemble contractile networks is required throughout cell physiology, yet direct biophysical mechanisms regulating non-muscle myosin 2 filament assembly in living cells are lacking. Here, we use a suite of dynamic, quantitative imaging approaches to identify deterministic factors that drive myosin filament appearance and amplification. We find that actin dynamics regulate myosin assembly, but that the static actin architecture plays a less clear role. Instead, remodeling of actin networks modulates the local myosin monomer levels and facilitates assembly through myosin:myosin-driven interactions. Using optogenetically controlled myosin, we demonstrate that locally concentrating myosin is sufficient to both form filaments and jump-start filament amplification and

SUBMITTER: Quintanilla MA 

PROVIDER: S-EPMC10866686 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature