Development of an ostrich-derived single-chain variable fragment (scFv) against PTPRN extracellular domain.

ABSTRACT: In type 1 diabetes, the immune system destroys pancreatic beta cells in an autoimmune condition. To overcome this disease, a specific monoclonal antibody that binds to pancreatic beta cells could be used for targeted immunotherapy. Protein tyrosine phosphatase receptor N (PTPRN) is one of the important surface antigen candidates. Due to its high sequence homology among mammals, so far, no single-chain monoclonal antibody has been produced against this receptor. In this study, we developed a novel single-chain variable fragment (scFv) against the PTPRN extracellular domain. To this aim, ostrich species was used as a host is far phylogenetically birds from mammals to construct a phage display library for the first time. An ostrich-derived scfv phage display library was prepared and biopannin

SUBMITTER: Dabiri H 

PROVIDER: S-EPMC10866909 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature