A colorimetric immunoassay for the detection of human vascular endothelial growth factor 165 (VEGF₁₆₅) based on anti-VEGF-iron oxide nanoparticle conjugation.

ABSTRACT: Vascular endothelial growth factor (VEGF) is an indispensable element in many physiological processes, while alterations in its level in the circulating system are signs of pathology-associated diseases. Therefore, its precise and selective detection is critical for clinical applications to monitor the progression of the pathology. In this study, an optical immunoassay biosensor was developed as a model study for detecting recombinant VEGF₁₆₅. The VEGF₁₆₅ sample was purified from recombinant Kluyveromyces lactis GG799 yeast cells. Indirect ELISA was used during the detection, wherein iron oxide nanoparticles (FeNPs) were utilized to obtain optical signals. The FeNPs were synthesized in the presence of lactose p-amino benzoic acid (LpAB). VEGF₁₆₅ antibody wa

SUBMITTER: Ceylan HK 

PROVIDER: S-EPMC10867064 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature