Bubble DNA tweezer: A triple-conformation sensor responsive to concentration-ratios.

ABSTRACT: DNA tweezers, with their elegant simplicity and flexibility, have been pivotal in biosensing and DNA computing. However, conventional tweezers are confined to a binary transformation pre/post target signal recognition, limiting them to presence/absence judgments. This study introduces bubble DNA tweezers (BDT), capable of three distinct conformations based on variable target signal ratios. In contrast to traditional compact tweezers, BDT features a looser structure centered around a non-complementary bubble domain located between the tweezer arms' connecting axis and target signal recognition jaws. This bubble triggers toehold-free DNA strand displacement, leading to three conformational changes at different target signal concentrations. BDT detects presence/absence and true concentration

SUBMITTER: Yao Y 

PROVIDER: S-EPMC10867447 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature