Exploring the potential human pathogenic bacteria in selected ready-to-eat leafy greens sold in Dhaka City, Bangladesh: Estimation of bacterial load and incidence.

ABSTRACT: This study was designed to investigate the presence of potential human pathogenic bacteria, bacterial load, and their incidence in ready-to-eat leafy greens viz., coriander, lettuce, and mint leaves sold at diverse marketplaces in Dhaka City. Multiple identification methods including cultural, morphological, biochemical, and molecular analysis were employed in the Plant Pathology Laboratory of Sher-e-Bangla Agricultural University to identify the human pathogenic bacteria. In molecular analysis, the DNA samples were put through PCR using bacterial primer 27F: AGAGTTTGATCMTGGCTGAG and universal primer 1942R: CGGTTACCTTGTTACGACTT. Initially, nine different bacterial genera viz. Bacillus, Escherichia, Pseudomonas, Neisseria, Klebsiella, Enterobacter, Shigella, Vibrio, and Staphyloco

SUBMITTER: Ferdous R 

PROVIDER: S-EPMC10867497 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature