Differential inflammation responses determine the variable phenotypes of epilepsy induced by GABRG2 mutations.

ABSTRACT:

Objective

To explore the mechanism involved in variable phenotypes of epilepsy models induced by γ-aminobutyric acid type A γ2 subunit (GABRG2) mutations.

Methods

The zebrafish carrying wild-type (WT) GABRG2, mutant GABRG2(P282S), GABRG2(F343L) and GABRG2(I107T) were established by Tol2kit transgenesis system and Gateway method. Behavioral analysis of different transgenic zebrafish was performed with the DanioVision Video-Track framework and the brain activity was analyzed by field potential recording with MD3000 Bio-signal Acquisition and Processing System. The transcriptome analysis was applied to detect the underlying mechanisms of variable phenotypes caused by different GABRG2 mutations.

Results

The established Tg(hGABRG2^P282S ) zebrafish showed hyperac

SUBMITTER: Sui J 

PROVIDER: S-EPMC10867793 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature