Versatile Capillary Cells for Handling Concentrated Samples in Analytical Ultracentrifugation.

ABSTRACT: In concentrated macromolecular dispersions, far-from-ideal intermolecular interactions determine the dispersion behaviors including phase transition, crystallization, and liquid-liquid phase separation. Here, we present a novel versatile capillary-cell design for analytical ultracentrifugation-sedimentation equilibrium (AUC-SE), ideal for studying samples at high concentrations. Current setups for such studies are difficult and unreliable to handle, leading to a low experimental success rate. The design presented here is easy to use, robust, and reusable for samples in both aqueous and organic solvents while requiring no special tools or chemical modification of AUC cells. The key and unique feature is the fabrication of liquid reservoirs directly on the bottom window of AUC cells, which c

SUBMITTER: Ong Q 

PROVIDER: S-EPMC10867799 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature