N-terminal truncation of PhaC_BP-M-CPF4 and its effect on PHA production.

ABSTRACT:

Background

Among the polyhydroxyalkanoate (PHA), poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate] [P(3HB-co-3HHx)] is reported to closely resemble polypropylene and low-density polyethylene. Studies have shown that PHA synthase (PhaC) from mangrove soil (PhaC_BP-M-CPF4) is an efficient PhaC for P(3HB-co-3HHx) production and N-termini of PhaCs influence its substrate specificity, dimerization, granule morphology, and molecular weights of PHA produced. This study aims to further improve PhaC_BP-M-CPF4 through N-terminal truncation.

Results

The N-terminal truncated mutants of PhaC_BP-M-CPF4 were constructed based on the information of the predicted secondary and tertiary structures using PSIPRED server and AlphaFold2 program, respectively. The

SUBMITTER: Neoh SZ 

PROVIDER: S-EPMC10867992 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature