The involvement of Neuregulin-1 in the process of facial nerve injury repair through the utilization of dental pulp stem cells.

ABSTRACT:

Background

Facial nerve injury often results in poor prognosis due to the challenging process of nerve regeneration. Neuregulin-1, a human calmodulin, is under investigation in this study for its impact on the reparative capabilities of Dental Pulp Stem Cells (DPSCs) in facial nerve injury.

Methods

Lentivirus was used to transfect and construct Neuregulin-1 overexpressed DPSCs. Various techniques assessed the effects of Neuregulin-1: osteogenic induction, lipid induction, Reverse Transcription Polymerase Chain Reaction, Western Blot, Cell Counting Kit-8 assay, wound healing, immunofluorescence, Phalloidin staining, nerve stem action potential, Hematoxylin-eosin staining, transmission electron microscopy, and immunohistochemistry.

Results

Neuregulin-1 effectively enhan

SUBMITTER: Yao L 

PROVIDER: S-EPMC10868091 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature