Tracking arboviruses, their transmission vectors and potential hosts by nanopore sequencing of mosquitoes.

ABSTRACT: The risk to human health from mosquito-borne viruses such as dengue, chikungunya and yellow fever is increasing due to increased human expansion, deforestation and climate change. To anticipate and predict the spread and transmission of mosquito-borne viruses, a better understanding of the transmission cycle in mosquito populations is needed. We present a pathogen-agnostic combined sequencing protocol for identifying vectors, viral pathogens and their hosts or reservoirs using portable Oxford Nanopore sequencing. Using mosquitoes collected in São Paulo, Brazil, we extracted RNA for virus identification and DNA for blood meal and mosquito identification. Mosquitoes and blood meals were identified by comparing cytochrome c oxidase I (COI) sequences against a curated Barcode of Life Data Syst

SUBMITTER: Mirza JD 

PROVIDER: S-EPMC10868619 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature