Design of intrinsically disordered proteins that undergo phase transitions with lower critical solution temperatures.

ABSTRACT: Many naturally occurring elastomers are intrinsically disordered proteins (IDPs) built up of repeating units and they can demonstrate two types of thermoresponsive phase behavior. Systems characterized by lower critical solution temperatures (LCST) undergo phase separation above the LCST whereas systems characterized by upper critical solution temperatures (UCST) undergo phase separation below the UCST. There is congruence between thermoresponsive coil-globule transitions and phase behavior whereby the theta temperatures above or below which the IDPs transition from coils to globules serve as useful proxies for the LCST / UCST values. This implies that one can design sequences with desired values for the theta temperature with either increasing or decreasing radii of gyration above the the

SUBMITTER: Zeng X 

PROVIDER: S-EPMC10868716 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature