IFI16 plays a critical role in avian reovirus induced cellular immunosuppression and suppresses virus replication.

ABSTRACT: Avian reovirus (ARV), which commonly induces viral arthritis or tenosynovitis and immunosuppression in chickens, is associated with the nonstructural protein p17 that plays a crucial role in viral replication and regulates cellular signaling pathways through its interaction with cellular proteins. In our previous study, we identified the host protein IFN-γ-inducible protein-16 (IFI16) as an interacting partner of ARV p17 through yeast two-hybrid screening. In the current study, we further confirmed the interaction between IFI16 and p17 protein using coimmunoprecipitation, glutathione S-transferase (GST)-pulldown assay, and laser confocal microscopy techniques. Additionally, we found that the amino acid of p17_61-119 is responsible for mediating the interaction with the HINa and H

SUBMITTER: Chengcheng Z 

PROVIDER: S-EPMC10869280 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature