Antibody and antibody fragments site-specific conjugation using new Q-tag substrate of bacterial transglutaminase.

ABSTRACT: During the last few years Antibody-Drug Conjugates (ADCs) have become one of the most active and very promising therapeutic weapons. Lessons learned from the traditional chemical conjugations (via lysine or cysteine residues of the antibodies) and the clinical studies of the developed ADCs have recently paved the way to the improvement of the conjugation technologies. Use of site-specific conjugation is considered as the promising path for improving the design and development of homogeneous ADCs with controlled Drug-Antibody ratio (DAR). Moreover, some of these conjugations can be applied to antibody fragments such as Fab, scfv and VHH for which random and chemical conjugation showed significant limitations. In this study, we identified a novel small peptide substrate (Q-tag) with high aff

SUBMITTER: El Alaoui M 

PROVIDER: S-EPMC10869684 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature