Identification of structural and regulatory cell-shape determinants in Haloferax volcanii.

ABSTRACT: Archaea play indispensable roles in global biogeochemical cycles, yet many crucial cellular processes, including cell-shape determination, are poorly understood. Haloferax volcanii, a model haloarchaeon, forms rods and disks, depending on growth conditions. Here, we used a combination of iterative proteomics, genetics, and live-cell imaging to identify mutants that only form rods or disks. We compared the proteomes of the mutants with wild-type cells across growth phases, thereby distinguishing between protein abundance changes specific to cell shape and those related to growth phases. The results identified a diverse set of proteins, including predicted transporters, transducers, signaling components, and transcriptional regulators, as important for cell-shape determination. Through pheno

SUBMITTER: Schiller H 

PROVIDER: S-EPMC10869688 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature