SIRT7 promotes the proliferation and migration of anaplastic thyroid cancer cells by regulating the desuccinylation of KIF23.

ABSTRACT:

Objective

This study was designed to investigate the regulatory effects of kinesin family member (KIF) 23 on anaplastic thyroid cancer (ATC) cell viability and migration and the underlying mechanism.

Methods

Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to analyze the levels of KIF23 in ATC cells. Besides, the effects of KIF23 and sirtuin (SIRT) 7 on the viability and migration of ATC cells were detected using cell counting kit-8, transwell and wound healing assays. The interaction between SIRT7 and KIF23 was evaluated by co-immunoprecipitation (Co-IP) assay. The succinylation (succ) of KIF23 was analyzed by western blot.

Results

The KIF23 expression was upregulated in ATC cells. Silencing of KIF23 suppressed the viability and migrati

SUBMITTER: Wu Y 

PROVIDER: S-EPMC10870498 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature