Genome-wide comparative methylation analysis reveals the fate of germ stem cells after surrogate production in teleost.

ABSTRACT:

Background

Surrogate production by germline stem cell transplantation is a powerful method to produce donor-derived gametes via a host, a practice known as surrogacy. The gametes produced by surrogates are often analysed on the basis of their morphology and species-specific genotyping, which enables conclusion to be drawn about the donor's characteristics. However, in-depth information, such as data on epigenetic changes, is rarely acquired. Germ cells develop in close contact with supporting somatic cells during gametogenesis in vertebrates, and we hypothesize that the recipient's gonadal environment may cause epigenetic changes in produced gametes and progeny. Here, we extensively characterize the DNA methylome of donor-derived sperm and their intergenerational effects in both in

SUBMITTER: Nayak R 

PROVIDER: S-EPMC10870548 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature