The m⁶A methylation and expression profiles of mouse neural stem cells after hypoxia/reoxygenation.

ABSTRACT:

Background

Ischemia-reperfusion injury to the central nervous system often causes severe complications. The activation of endogenous neural stem cells (NSCs) is considered a promising therapeutic strategy for nerve repair. However, the specific biological processes and molecular mechanisms of NSC activation remain unclear, and the role of N6-methyladenosine (m⁶A) methylation modification in this process has not been explored.

Methods

NSCs were subjected to hypoxia/reoxygenation (H/R) to simulate ischemia-reperfusion in vivo. m⁶A RNA methylation quantitative kit was used to measure the total RNA m⁶A methylation level. Quantitative real-time PCR was used to detect methyltransferase and demethylase mRNA expression levels. Methylated RNA immunopr

SUBMITTER: Zhang S 

PROVIDER: S-EPMC10870567 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature