Dual-Angle Interferometric Scattering Microscopy for Optical Multiparametric Particle Characterization.

ABSTRACT: Traditional single-nanoparticle sizing using optical microscopy techniques assesses size via the diffusion constant, which requires suspended particles to be in a medium of known viscosity. However, these assumptions are typically not fulfilled in complex natural sample environments. Here, we introduce dual-angle interferometric scattering microscopy (DAISY), enabling optical quantification of both size and polarizability of individual nanoparticles (radius <170 nm) without requiring a priori information regarding the surrounding media or super-resolution imaging. DAISY achieves this by combining the information contained in concurrently measured forward and backward scattering images through twilight off-axis holography and interferometric scattering (iSCAT). Going beyond particle

SUBMITTER: Olsen E 

PROVIDER: S-EPMC10870763 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature