Distinct structural motifs are necessary for targeting and import of Tim17 in Trypanosoma brucei mitochondrion.

ABSTRACT: Nuclear-encoded mitochondrial proteins are correctly translocated to their proper sub-mitochondrial destination using location-specific mitochondrial targeting signals and via multi-protein import machineries (translocases) in the outer and inner mitochondrial membranes (TOM and TIMs, respectively). However, targeting signals of multi-pass Tims are less defined. Here, we report the characterization of the targeting signals of Trypanosoma brucei Tim17 (TbTim17), an essential component of the most divergent TIM complex. TbTim17 possesses a characteristic secondary structure including four predicted transmembrane (TM) domains in the center with hydrophilic N- and C-termini. After examining mitochondrial localization of various deletion and site-directed mutants of TbTim17 in T. bruc

SUBMITTER: Darden C 

PROVIDER: S-EPMC10871166 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature