Biochemical fractionation of human α-Synuclein in a Drosophila model of synucleinopathies.

ABSTRACT: Synucleinopathies are a group of central nervous system pathologies that are characterized by neuronal accumulation of misfolded and aggregated α-synuclein in proteinaceous depositions known as Lewy Bodies (LBs). The transition of α-synuclein from its physiological to pathological form has been associated with several post-translational modifications such as phosphorylation and an increasing degree of insolubility, which also correlate with disease progression in postmortem specimens from human patients. Neuronal expression of α-synuclein in model organisms, including Drosophila melanogaster, has been a typical approach employed to study its physiological effects. Biochemical analysis of α-synuclein solubility via high-speed ultracentrifugation with buffers of increasing detergent s

SUBMITTER: Imomnazarov K 

PROVIDER: S-EPMC10871193 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature