Peptide-based capture-and-release purification of extracellular vesicles and statistical algorithm enabled quality assessment.

ABSTRACT: Circulating extracellular vesicles (EVs) have gained significant attention for discovering tumor biomarkers. However, isolating EVs with well-defined homogeneous populations from complex biological samples is challenging. Different isolation methods have been found to derive different EV populations carrying different molecular contents, which confounds current investigations and hinders subsequent clinical translation. Therefore, standardizing and building a rigorous assessment of isolated EV quality associated with downstream molecular analysis is essential. To address this need, we introduce a statistical algorithm (ExoQuality Index, EQI) by integrating multiple EV characterizations (size, particle concentration, zeta potential, total protein, and RNA), enabling direct EV quality assess

SUBMITTER: Greenberg ZF 

PROVIDER: S-EPMC10871196 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature