Preservation of memory B cell homeostasis in an individual producing broadly neutralising antibodies against HIV-1.

ABSTRACT: Immunological determinants favouring emergence of broadly neutralising antibodies are crucial to the development of HIV-1 vaccination strategies. Here, we combined RNAseq and B cell cloning approaches to isolate a broadly neutralising antibody (bnAb) ELC07 from an individual living with untreated HIV-1. Using single particle cryogenic electron microscopy (cryo-EM), we show that the antibody recognises a conformational epitope at the gp120-gp41 interface. ELC07 binds the closed state of the viral glycoprotein causing considerable perturbations to the gp41 trimer core structure. Phenotypic analysis of memory B cell subsets from the ELC07 bnAb donor revealed a lack of expected HIV-1-associated dysfunction, specifically no increase in CD21^-/CD27^- cells was observed whilst

SUBMITTER: Griffith S 

PROVIDER: S-EPMC10871235 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature