MAGIC matrices: freeform bioprinting materials to support complex and reproducible organoid morphogenesis.

ABSTRACT: Organoids are powerful models of tissue physiology, yet their applications remain limited due to their relatively simple morphology and high organoid-to-organoid structural variability. To address these limitations we developed a soft, composite yield-stress extracellular matrix that supports optimal organoid morphogenesis following freeform 3D bioprinting of cell slurries at tissue-like densities. The material is designed with two temperature regimes: at 4 °C it exhibits reversible yield-stress behavior to support long printing times without compromising cell viability. When transferred to cell culture at 37 °C, the material cross-links and exhibits similar viscoelasticity and plasticity to basement membrane extracts such as Matrigel. We first characterize the rheological properties of MA

SUBMITTER: Graham AJ 

PROVIDER: S-EPMC10871257 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature