DeepSLICEM: Clustering CryoEM particles using deep image and similarity graph representations.

ABSTRACT: Finding the 3D structure of proteins and their complexes has several applications, such as developing vaccines that target viral proteins effectively. Methods such as cryogenic electron microscopy (cryo-EM) have improved in their ability to capture high-resolution images, and when applied to a purified sample containing copies of a macromolecule, they can be used to produce a high-quality snapshot of different 2D orientations of the macromolecule, which can be combined to reconstruct its 3D structure. Instead of purifying a sample so that it contains only one macromolecule, a process that can be difficult, time-consuming, and expensive, a cell sample containing multiple particles can be photographed directly and separated into its constituent particles using computational methods. Previous

SUBMITTER: Palukuri MV 

PROVIDER: S-EPMC10871265 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature