TNF promoter hypomethylation is associated with mucosal inflammation in IBD and anti-TNF response.

ABSTRACT:

Background and aims

Inflammatory Bowel Diseases (IBD) are chronic inflammatory conditions influenced heavily by environmental factors. DNA methylation is a form of epigenetic regulation linking environmental stimuli to gene expression changes and inflammation. Here, we investigated how DNA methylation of the TNF promoter differs between inflamed and uninflamed mucosa of IBD patients, including anti-TNF responders and non-responders.

Methods

We obtained mucosal biopsies from 200 participants (133 IBD and 67 controls) and analyzed TNF promoter methylation using bisulfite sequencing, comparing inflamed with uninflamed segments, in addition to paired inflamed/uninflamed samples from individual patients. We conducted similar analyses on purified intestinal epithelia

SUBMITTER: Levic DS 

PROVIDER: S-EPMC10871362 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature