RCRUX: A Rapid and Versatile Tool for Generating Metabarcoding Reference libraries in R.

ABSTRACT: The sequencing revolution requires accurate taxonomic classification of DNA sequences. Key to making accurate taxonomic assignments are curated, comprehensive reference barcode databases. However, the generation and curation of such databases has remained challenging given the large and continuously growing volumes of both DNA sequence data and novel reference barcode targets. Monitoring and research applications require a greater diversity of specialized gene regions and targeted taxa then are currently curated by professional staff. Thus there is a growing need for an easy to implement computational tool that can generate comprehensive metabarcoding reference libraries for any bespoke locus. We address this need by reimagining CRUX from the Anacapa Toolkit and present the rCRUX package i

SUBMITTER: Curd EE 

PROVIDER: S-EPMC10871694 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature