Dissection of an impact of VDR and RXRA on the genomic activity of 1,25(OH)₂D₃ in A431 squamous cell carcinoma.

ABSTRACT:

Background

Human skin is the natural source, place of metabolism, and target for vitamin D₃. The classical active form of vitamin D₃, 1,25(OH)₂D₃, expresses pluripotent properties and is intensively studied in cancer prevention and therapy. To define the specific role of vitamin D₃ receptor (VDR) and its co-receptor retinoid X receptor alpha (RXRA) in genomic regulation, VDR or RXRA genes were silenced in the squamous cell carcinoma cell line A431 and treated with 1,25(OH)₂D₃ at long incubation time points 24 h/72 h. Extending the incubation time of A431 WT (wild-type) cells with 1,25(OH)₂D₃ resulted in a two-fold increase in DEGs (differentially expressed genes) and a change in the a

SUBMITTER: Olszewska AM 

PROVIDER: S-EPMC10872374 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature