Chronic brain functional ultrasound imaging in freely moving rodents performing cognitive tasks.

ABSTRACT:

Background

Functional ultrasound imaging (fUS) is an emerging imaging technique that indirectly measures neural activity via changes in blood volume. Chronic fUS imaging during cognitive tasks in freely moving animals faces multiple exceptional challenges: performing large durable craniotomies with chronic implants, designing behavioral experiments matching the hemodynamic timescale, stabilizing the ultrasound probe during freely moving behavior, accurately assessing motion artifacts, and validating that the animal can perform cognitive tasks while tethered.

New method

We provide validated solutions for those technical challenges. In addition, we present standardized step-by-step reproducible protocols, procedures, and data processing pipelines. Finally, we present proof-of-

SUBMITTER: El Hady A 

PROVIDER: S-EPMC10872377 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature