Vertebrate Nup53 interacts with the nuclear lamina and is required for the assembly of a Nup93-containing complex.

ABSTRACT: The nuclear pore complex (NPC) is an evolutionarily conserved structure that mediates exchange of macromolecules across the nuclear envelope (NE). It is comprised of approximately 30 proteins termed nucleoporins that are each present in multiple copies. We have investigated the function of the human nucleoporin Nup53, the ortholog of Saccharomyces cerevisiae Nup53p. Both cell fractionation and in vitro binding data suggest that Nup53 is tightly associated with the NE membrane and the lamina where it interacts with lamin B. We have also shown that Nup53 is capable of physically interacting with a group of nucleoporins including Nup93, Nup155, and Nup205. Consistent with this observation, depletion of Nup53 using small interfering RNAs causes a decrease in the cellular levels of these nucleo

SUBMITTER: Hawryluk-Gara LA 

PROVIDER: S-EPMC1087243 | biostudies-literature | 2005 May

REPOSITORIES: biostudies-literature